Coding

Part:BBa_K401001:Design

Designed by: Daniel Tamarit   Group: iGEM10_Valencia   (2010-10-21)


NMGR526 (construction)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 122
    Illegal PstI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 122
    Illegal PstI site found at 2323
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 122
    Illegal PstI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 122
    Illegal PstI site found at 2323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2009


Design Notes

The NMGR526 construction can be amplified using the following primers: forward ATGTCGGATTCAAAC and reverse TCCTGCAGTGGCTTG.


Source

The NM fragment comes from the protein Sup35. The GR526 fragment comes from the protein GR. To read more about this construction, read Li and Lindquist (2000), Science, 287: 661-664.

References